cloning and expression of recombinant plasmid containing p36/lack gene of leishmania infantum iranian strain.
saloomeh shirali dept. of biology, faculty of basic science, ahvaz branch, islamic azad university, ahvaz, iran and dept. of parasitology, faculty of veterinary medicine, university of tehran, tehran, iran., hamidreza haddadzadeh dept. of parasitology, faculty of veterinary medicine, university of tehran, tehran, iran., mehdi mohebali dept. of medical parasitology and mycology, school of public health, tehran university of medical sciences, iran and center for research of endemic parasites of iran (crepi), tehran university of medical sciences, tehran, iran., bahram kazemi cellular and molecular biology research center, shahid beheshti university of medical sciences, tehran, iran and dept. of biotechnology, school of medicine, shahid beheshti university of medical sciences, tehran, iran., narges amini dept. of parasitology, faculty of veterinary medicine, university of tehran, tehran, iran.
چکیده(به انگلیسی؛ چکیدهی فارسی در دسترس نیست)
background : there are several methods, such as vaccination, to control visceral leishmaniasis. although there is no efficient vaccine, it seem dna vaccination with stimulates both cellular and humoral immunity apparently is the best way. the aim of this study was cloning and expression of lack gene, a 36kd protein, as a can-didate protein for vaccination against iranian l.infantum. methods: iranian strain of l. infantum [mcan/ir/07/moheb-gh] was used as a template for pcr to amplify lack gene. the lack gene was cloned in ptz57r/t vector and after confirmation it was digested by restriction enzymes (bamh1) and cloned in pcdna3.1 expression vector. recombinant plasmid was extracted and analyzed by sequencing, restriction digestion analysis and pcr reac-tion. the pc- lack recombinant plasmid was purified from transformed e.coli (dh5α) and its expression was analyzed by sds-page and western blot. results: the results of sequencing, restriction digestion analysis and pcr reaction revealed that lack gene was cloned correctly in pcdna3.1 vector and the results of sds page and western blot emphasized that lack protein of iranian l. in-fantum is a well-expressed protein. conclusion: we amplified, cloned and expressed iranian l. infantum lack genes successfully.
سند علمی۲۰۱۵Iranian journal of parasitologySaloomeh SHIRALI, Hamidreza HADDADZADEH, Mehdi MOHEBALI, Bahram KAZEMI
BACKGROUND There are several methods, such as vaccination, to control visceral leishmaniasis. Although there is no efficient vaccine, it seem DNA vaccination with stimulates both cellular and humoral immunity apparently is the best way. The aim of this study was cloning and expre
سند علمی۲۰۱۵Revista brasileira de parasitologia veterinaria = Brazilian journal of veterinary parasitology : Orgao Oficial do Colegio Brasileiro de Parasitologia VeterinariaMárcia Mariza Gomes Jusi, Trícia Maria Ferreira de Sousa Oliveira, Andréa Cristina Higa Nakaghi, Marcos Rogério André
This study aimed to express a recombinant A2 family protein of Leishmania chagasi, Jaboticabal strain; test this protein as an antigen in serological assays; and investigate its antigenicity and immunogenicity. A protein coded by an allele of the A2 gene isolated from L. chagasi
سند علمی۱۹۸۴Journal of bacteriologyS A Lacks, S S Springhorn
Plasmid transfer via the transformation pathway of Streptococcus pneumoniae was weakly restricted by the DpnI or DpnII restriction endonuclease, either of which gave a reduction only to 0.4, compared with phage infection, which was restricted to 10(-5). The greater sensitivity of
سند علمی۲۰۰۹Journal of biomedicine & biotechnologyXiaolin Li, Guozhong Jiang, Dan Wu, Xiuli Wang
AIM To construct a recombinant eukaryotic expression plasmid containing human calcitonin (hCT) gene and express the gene in murine fibroblast NIH3T3 cells. MATERIALS AND METHODS A murine Igkappa-chain leader sequence and hCT gene were synthesized and cloned into pCDNA3.0 to form