molecular recognition of human papilloma virus (hpv) using proprietary pcr method based on l1 gene and the evaluation of its frequency in tissue samples from patients with cervical cancer

روح اله درستکار, roohollah dorostkar applied virology research center, baqiyatallah university of medical sciences, tehran, iranمرکز تحقیقات ویروس شناسی کاربردی، دانشگاه و پژوهشگاه علوم پزشکی بقیه الله الاعظم (عج)، تهران، ایران محمدصادق هاشم زاده, mohammad sadegh hashemzadeh applied virology research center, baqiyatallah university of medical sciences, tehran, iranمرکز تحقیقات ویروس شناسی کاربردی، دانشگاه و پژوهشگاه علوم پزشکی بقیه الله الاعظم (عج)، تهران، ایرانسازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (baqiyatallah university of medical sciences) مهدی تات, mahdi tat applied virology research center, baqiyatallah university of medical sciences, tehran, iranمرکز تحقیقات ویروس شناسی کاربردی، دانشگاه و پژوهشگاه علوم پزشکی بقیه الله الاعظم (عج)، تهران، ایرانسازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (baqiyatallah university of medical sciences) محمدرضا شفاعتی, mohammad raza shafaati applied virology research center, baqiyatallah university of medical sciences, tehran, iranمرکز تحقیقات ویروس شناسی کاربردی، دانشگاه و پژوهشگاه علوم پزشکی بقیه الله الاعظم(عج)، تهران، ایرانسازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (baqiyatallah university of medical sciences) محمد نجاراصل, mohammad najarasl applied virology research center, baqiyatallah university of medical sciences, tehran, iranمرکز تحقیقات ویروس شناسی کاربردی، دانشگاه و پژوهشگاه علوم پزشکی بقیه الله الاعظم (عج)، تهران، ایرانسازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (baqiyatallah university of medical sciences) سمانه ظهیری یگانه, samaneh zahiri yeganeh applied virology research center, baqiyatallah university of medical sciences, tehran, iranمرکز تحقیقات ویروس شناسی کاربردی، دانشگاه و پژوهشگاه علوم پزشکی بقیه الله الاعظم(عج)، تهران، ایرانسازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (baqiyatallah university of medical sciences)

چکیده(به انگلیسی؛ چکیده‌ی فارسی در دسترس نیست)

background: in 1970, human papillomavirus (hpv) was introduced as the main etiologic factor of cervical carcinoma. since there is no possibility of detecting the virus and its subtypes using serological methods and cell culture, the molecular methods such as pcr have particular importance in accurate, early and definite diagnosis of the virus. so, in this research, our goal is to use a proprietary pcr assay based on l1 gene of human papillomavirus for molecular recognition of hpv and to evaluate its prevalence in patient samples. materials and methods: in this experimental study, after collecting of samples from malignant cervical lesions, the viral dna was extracted from paraffin blocks of 50 clinical samples and pcr was done by specific primers for l1 gene of human papillomavirus in all samples. after the analysis of pcr products by 2% agarose gel electrophoresis, sensitivity and specificity of the test were also evaluated. results: among 50 patient samples, 33 cases were confirmed to be positive for hpv infection and 17 cases were negative, showing high frequency of hpv in this patient population (about 66%). the results of specificity assay were positive for papilloma samples and sensitivity of the assay was 20 copies of recombinant construct containing l1 per reaction. conclusion: this study showed that pcr by specific primers for l1 gene of human papilloma virus is a proper and accurate method for detection of this virus and the results confirm the previous reports of correlation between hpv and cervical carcinoma.