مقاله

application of sybr-green real time rt-pcr for quantification of human immunodeficiency virus type-1 (hiv-1) and comparison with cobas amplicor test

saeid amel jamehdar, farzaneh sabahi, mahdi forouzandeh, mahboubeh haji abdolbaghi, anooshiravan kazemnejad

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چکیده(به انگلیسی؛ چکیده‌ی فارسی در دسترس نیست)

objective: in this study, a sybr green real-time rt-pcr assay for quantification of hiv-1 viral rna was developed. materials and methods: this assay was performed based on amplification of the pol region of hiv-1 and product analysis by an abi 7500 system. we quantified hiv-1 viral load in 26 seropositive patients by this system and the data were subsequently compared with results obtained with a reference technique represented by cobas amplicor hiv-1 monitor test. results: the results demonstrated that this technique could detecte up to 500 hiv-1 rna copies/ml of plasma. the linearity of this approach was conserved over a wide range of hiv-1 copy numbers (5×102-5×109). since no positive signal was observed in seronegative volunteers, the specificity of the test was calculated as 100%. comparison of the results with those obtained by the reference quantification method, revealed a significant correlation between the results (r2= 0.95). conclusion: on the basis of the most recent recorded cases for hiv-1 infection and aids in iran, the prevalence of this disease is rising rapidly and the situation has been called to be alarming by national health representatives. determination of hiv-1 viral load in plasma has been considered as the most effective single prediction tool of clinical outcome. indeed, the development and stabilization of hiv-1 rna assays have given physicians a unique tool for monitoring hiv-1 patients treated with antiviral drugs. in this study, we have developed a sybr-green real time rt-pcr assay for quantitative analysis of hiv-1 in infected patients. since a synthetic rna standard was used in this assay, the upper limit of detection was detected to be higher than the standard test (5×10 9 versus 7.5×10 5). this can be important in patients with acute high viral load infections. reproducibility was assessed by intra assay and inter assay analysis. coefficient of variations ct, in reproducibility tests for intra assay and inter assay variability were less than

نویسندگان و وابستگی سازمانی
  1. saeid amel jamehdar — department of virology, school of medical sciences, tarbiat modares university, tehran, iran
  2. farzaneh sabahi — department of virology, school of medical sciences, tarbiat modares university, tehran, iran
  3. mahdi forouzandeh — department of biotechnology, school of medical sciences, tarbiat modares university, tehran, iran
  4. mahboubeh haji abdolbaghi — department of infectious diseases, aids research center of iran, faculty of medicine, tehran university of medical sciences, tehran, iran
  5. anooshiravan kazemnejad — department of biostatistics, school of medical sciences, tarbiat modares university, tehran, iran

اسناد مشابه

  • Optimization of the Sybr Green real time PCR for the detection of Human Herpes Virus type 6 (HHV-6).

    سند علمی۲۰۰۸Revista do Instituto de Medicina Tropical de Sao PauloCynthia Liliane Motta do Canto, Laura Massami Sumita, Adriana Freire Machado, Adriana Tateno

    HHV-6 is the etiological agent of Exanthem subitum which is considered the sixth most frequent disease in infancy. In immuno-compromised hosts, reactivation of latent HHV-6 infection may cause severe acute disease. We developed a Sybr Green Real Time PCR for HHV-6 and compared th

  • multiplex sybr green real-time pcr assay for detection of respiratory viruses

    مقالهjundishapur journal of microbiologymozhdeh sultani virology department, school of public health, tehran university of medical sciences, tehran, ir iran, talat mokhtari azad virology department, school of public health, tehran university of medical sciences, tehran, ir iran, mohammadreza eshragian virology department, school of public health, tehran university of medical sciences, tehran, ir iran, azadeh shadab virology department, school of public health, tehran university of medical sciences, tehran, ir iran

    conclusions multiplex sybr green real-time pcr is a rapid and relatively inexpensive method for detection of respiratory viruses. results application of the multiplex sybr green real-time pcr in clinical samples from 172 patients in a one-year study resulted in detection of 19 (1

  • Multiplex SYBR Green Real-Time PCR Assay for Detection of Respiratory Viruses

    سند علمی۲۰۱۵Jundishapur journal of microbiologyMozhdeh Sultani, Talat Mokhtari Azad, Mohammadreza Eshragian, Azadeh Shadab

    BACKGROUND It is often difficult for a physician to distinguish between viral and bacterial causes of respiratory infections and this may result in overuse of antibiotics. In many cases of community-acquired respiratory infections, clinicians treat patients empirically. The devel

  • Molecular Detection of Rabies by SYBR Green Real Time PCR

    سند علمی۲۰۱۲A Kaw, C K Singh, N K Sood

    The present study was envisaged to compare the sensitivity of SYBR Green real time PCR with immunofluorescence PCR for diagnosis of rabies. SYBR Green real time PCR technique was applied on brain samples collected from 39 rabies suspected animals. Sensitivity of SYBR Green techni

  • Comparison between two standardized cultural methods and 24 hour duplex SYBR green real-time PCR assay for Salmonella detectionin meat samples.

    سند علمی۲۰۱۱The new microbiologicaElisabetta Delibato, Alfonsina Fiore, Fabrizio Anniballi, Bruna Auricchio

    Food-borne diseases caused by Salmonella represent a worldwide public health problem. Salmonella must be absent in an established amount depending on the kind of the product and usually cultural methods have to be applied to evaluate the compliance of the products. ISO 6579:2002

  • development and comparison of conventional pcr and sybr green real time pcr for detection of aggregatibacter actinomycetemcomitans and tannerella forsythensis

    مقالهjundishapur journal of microbiologymohammad soleimani department of microbiology, qom branch, islamic azad university, qom, ir iran; department of microbiology, qom branch, islamic azad university, qom, ir iran, p.o. box: 37185/364. tel:+98-2517780001, fax: +98-2517780001, mohammad reza zolfaghari department of microbiology, qom branch, islamic azad university, qom, ir iran, abbas morovvati department of microbiology, qom branch, islamic azad university, qom, ir iran

    background aggregatibacter actinomycetemcomitans and tannerella forsythensis are two major pathogens in destructive periodontal disease in humans. the detection of these bacteria is needed for diagnosis and management of the mentioned diseases. objectives we aimed to develop and